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ml-si3  (MedChemExpress)


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    Structured Review

    MedChemExpress ml-si3
    Ml Si3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ml+si3/ML-SI3/custom%40hy-139426%4010%2E64898%2F2026%2E06%2E26%2E734916
    Average 94 stars, based on 17 article reviews
    ml-si3 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Live Cell Imaging:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Transfection:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Fluorescence:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Expressing:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Translocation Assay:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Staining:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Comparison:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Whisker Assay:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Control:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Patch Clamp:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Isolation:

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).

    Article Title: MONNA alleviates MPTP-induced Parkinson's disease in zebrafish by activating TFEB dependently on ER Calcium.
    Article Snippet: A-synuclein aggregation is a biomarker of Parkinson’s disease (PD) whose feature is the progressive loss of dopaminergic neuron in the middle brain.. The removal of a-synuclein aggregation through autophagy-lysosome pathway is a promising strategy for PD treatment.. Transcription factor EB (TFEB) is a master regulator of autophagic and lysosomal biogenesis and function.

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites
    Article Snippet: The following reagents were used in this study: YM-201636 (Cat. HY-13228/ MCE), Bafilomycin (Cat. BML-CM110-0100/ enzo live sciences), Halt phosphatase inhibitor (Cat. 78420/ Thermo Fisher Scientific), Biotin (Cat. B450/ Sigma), Cycloheximide (Cat. C1988-1G/ Sigma), LysotrackerTM Deep Red (Cat. L12492/ ThermoFisher), Magic Red Cathepsin B Kit (Cat. ICT938/ Bio-Rad), MitoTracker Deep Red FM (Cat. M22426/ ThermoFisher), Filipin complex ready-made solution (Cat. SAE0088/ Sigma), CellMask Green Actin Tracking Stain (Cat. A57243/ ThermoFisher), Hoechst 33342 Fluorescent Nucleic Acid Stain (Cat# 639/ ImmunoChemistry technologies).

    Article Title: Nortriptyline Inhibits Lysosomal Exocytosis‐Mediated SASP During Gastric Cancer Progression via Targeting HOXA1‐PITX2 Phase Separation
    Article Snippet: Cells were propagated in a humidified incubator at 37°C and 5% CO 2 , using RPMI 1640 medium prepared with 10% fetal bovine serum (Sigma, St. Louis, MO), and treated as specified with vacuolin‐1 (673000, Sigma), ionomycin (407951, Sigma), ML‐SI3 (MedChemExpress, Monmouth Junction, NJ), ML‐SA1 (MedChemExpress), 1,6‐hexanediol (88571, Sigma), or Nor (N‐907, Sigma).

    Article Title: Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity
    Article Snippet: MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

    Article Title: TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells.
    Article Snippet: For cellular treatments the following reagents were used: Torin 1 (Tocris, 4247), Mitomycin C (Merck (Roche), 10107409001), ML-SA1 (Bio-Techne, 4746), ML-SA5 (Enamine Ltd., 2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995).



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    (A) Schematic representation of calcium transport during ELR. (B) Schematic representation of the assay used to inhibit the lysosomal calcium exporter (TRPML1) or the mitochondrial calcium importer (VDAC) to assess its effect on ELR. (C) Live-cell imaging of cells co-transfected with LAMP1-GFP (green) and the mitochondrial calcium sensor mt-RCAMP1h (magenta). ELR was initiated with 2 h YM201636 treatment and washout. Cells were imaged for 30 min at 15-min intervals. D) Mean fluorescence intensity of mt-RCAMP1h (magenta) was quantified and plotted for 0 min, 15 min and 30 min recovery timepoints. Each dot represents the gray value of an individual cell. The plot shows the mean fluorescence intensity of 30 cells from n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ****P = 0.5257 × 10 −4 (YM201636 vs. 15 min recovery), ****P = 0.03522 × 10 −6 (YM201636 vs. 30 min recovery), ns P > 0.9473 (15 min recovery vs. 30 min recovery). E) Live-cell imaging of cells expressing LAMP1-GFP and treated with YM201636 together with the TRPML1 inhibitor ML-SI3. Cells were subsequently washed and recovered in media containing only ML-SI3 to test the effect of ML-SI3 alone, as described in (B). ROIs show the LAMP1-positive compartments in representative parts of cells. (F) Quantification of the average area of LAMP1-positive structures for (E). Each dot represents the average area per 100 µm² ROI. The plot shows the mean area from a total of 90 ROIs from 30 cells across n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ns P > 0.9 ×10 −14 ( 2 h YM201636 + 30 min recovery vs. 2 h YM201636 and ML-SI3 + 30 min recovery), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 + 30 min recovery with ML-SI3), ****P = 0.691 × 10 −12 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and ML-SI3 + 15 min recovery), ns P > 0.9 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and ML-SI3 + 30 min recovery), ****P = 0.4885 ×10 −4 (2 h YM201636 + 30 min recovery with ML-SI3 vs. 2 h YM201636 and ML-SI3 + 15 min recovery), ****P = < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery with ML-SI3 vs. 2 h YM201636 and ML-SI3 + 30 min recovery). (G) Live-cell imaging of cells expressing LAMP1-GFP and treated with YM201636 together with the VDAC2/3 inhibitor erastin. Cells were subsequently washed and recovered in media containing only erastin to test the effect of erastin alone, as described in (B). ROIs show the LAMP1-positive compartments in representative parts of cells. (H) Quantification of the average area of LAMP1-positive structures for (G). Each dot represents the average area per 100 µm² ROI. The plot shows the mean area from a total of 90 ROIs from 30 cells across n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ns P > 0.9 ×10 −14 ( 2 h YM201636 + 30 min recovery vs. 2 h YM201636 and Erastin + 30 min recovery), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 + 30 min recovery with Erastin), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and Erastin + 15 min recovery), ns P > 0.9 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and Erastin + 30 min recovery), **P = 0.00103 (2 h YM201636 + 30 min recovery with Erastin vs. 2 h YM201636 and Erastin + 15 min recovery), ****P = < 0.9 ×10 −14 (2 h YM201636 + 30 min recovery with Erastin vs. 2 h YM201636 and Erastin + 30 min recovery). (I) Live-cell imaging of cells expressing LAMP1-GFP and treated with YM201636 together with the VDAC1 inhibitor VBIT-4. Cells were subsequently washed and recovered in media containing only VBIT-4 to test the effect of VBIT-4 alone, as described in (B). ROIs show the LAMP1-positive compartments in representative parts of cells. (J) Quantification of the average area of LAMP1-positive structures for (I). Each dot represents the average area per 100 µm² ROI. The plot shows the mean area from a total of 90 ROIs from 30 cells across n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ns P > 0.9 ×10 −14 ( 2 h YM201636 + 30 min recovery vs. 2 h YM201636 and VBIT-4 + 30 min recovery), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 + 30 min recovery with VBIT-4), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and VBIT-4 + 15 min recovery), ns P =0.2364 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and VBIT-4 + 30 min recovery), **P = 0.29 ×10 −4 (2 h YM201636 + 30 min recovery with VBIT-4 vs. 2 h YM201636 and VBIT-4 + 15 min recovery), ****P = < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery with VBIT-4 vs. 2 h YM201636 and VBIT-4 + 30 min recovery).

    Journal: bioRxiv

    Article Title: Ca 2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites

    doi: 10.64898/2026.01.30.702748

    Figure Lengend Snippet: (A) Schematic representation of calcium transport during ELR. (B) Schematic representation of the assay used to inhibit the lysosomal calcium exporter (TRPML1) or the mitochondrial calcium importer (VDAC) to assess its effect on ELR. (C) Live-cell imaging of cells co-transfected with LAMP1-GFP (green) and the mitochondrial calcium sensor mt-RCAMP1h (magenta). ELR was initiated with 2 h YM201636 treatment and washout. Cells were imaged for 30 min at 15-min intervals. D) Mean fluorescence intensity of mt-RCAMP1h (magenta) was quantified and plotted for 0 min, 15 min and 30 min recovery timepoints. Each dot represents the gray value of an individual cell. The plot shows the mean fluorescence intensity of 30 cells from n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ****P = 0.5257 × 10 −4 (YM201636 vs. 15 min recovery), ****P = 0.03522 × 10 −6 (YM201636 vs. 30 min recovery), ns P > 0.9473 (15 min recovery vs. 30 min recovery). E) Live-cell imaging of cells expressing LAMP1-GFP and treated with YM201636 together with the TRPML1 inhibitor ML-SI3. Cells were subsequently washed and recovered in media containing only ML-SI3 to test the effect of ML-SI3 alone, as described in (B). ROIs show the LAMP1-positive compartments in representative parts of cells. (F) Quantification of the average area of LAMP1-positive structures for (E). Each dot represents the average area per 100 µm² ROI. The plot shows the mean area from a total of 90 ROIs from 30 cells across n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ns P > 0.9 ×10 −14 ( 2 h YM201636 + 30 min recovery vs. 2 h YM201636 and ML-SI3 + 30 min recovery), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 + 30 min recovery with ML-SI3), ****P = 0.691 × 10 −12 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and ML-SI3 + 15 min recovery), ns P > 0.9 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and ML-SI3 + 30 min recovery), ****P = 0.4885 ×10 −4 (2 h YM201636 + 30 min recovery with ML-SI3 vs. 2 h YM201636 and ML-SI3 + 15 min recovery), ****P = < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery with ML-SI3 vs. 2 h YM201636 and ML-SI3 + 30 min recovery). (G) Live-cell imaging of cells expressing LAMP1-GFP and treated with YM201636 together with the VDAC2/3 inhibitor erastin. Cells were subsequently washed and recovered in media containing only erastin to test the effect of erastin alone, as described in (B). ROIs show the LAMP1-positive compartments in representative parts of cells. (H) Quantification of the average area of LAMP1-positive structures for (G). Each dot represents the average area per 100 µm² ROI. The plot shows the mean area from a total of 90 ROIs from 30 cells across n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ns P > 0.9 ×10 −14 ( 2 h YM201636 + 30 min recovery vs. 2 h YM201636 and Erastin + 30 min recovery), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 + 30 min recovery with Erastin), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and Erastin + 15 min recovery), ns P > 0.9 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and Erastin + 30 min recovery), **P = 0.00103 (2 h YM201636 + 30 min recovery with Erastin vs. 2 h YM201636 and Erastin + 15 min recovery), ****P = < 0.9 ×10 −14 (2 h YM201636 + 30 min recovery with Erastin vs. 2 h YM201636 and Erastin + 30 min recovery). (I) Live-cell imaging of cells expressing LAMP1-GFP and treated with YM201636 together with the VDAC1 inhibitor VBIT-4. Cells were subsequently washed and recovered in media containing only VBIT-4 to test the effect of VBIT-4 alone, as described in (B). ROIs show the LAMP1-positive compartments in representative parts of cells. (J) Quantification of the average area of LAMP1-positive structures for (I). Each dot represents the average area per 100 µm² ROI. The plot shows the mean area from a total of 90 ROIs from 30 cells across n = 3 biological replicates; Kruskal-Wallis test with Dunn’s multiple comparisons: ns P > 0.9 ×10 −14 ( 2 h YM201636 + 30 min recovery vs. 2 h YM201636 and VBIT-4 + 30 min recovery), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 + 30 min recovery with VBIT-4), ****P < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and VBIT-4 + 15 min recovery), ns P =0.2364 (2 h YM201636 + 30 min recovery vs. 2 h YM201636 and VBIT-4 + 30 min recovery), **P = 0.29 ×10 −4 (2 h YM201636 + 30 min recovery with VBIT-4 vs. 2 h YM201636 and VBIT-4 + 15 min recovery), ****P = < 0.1 ×10 −14 (2 h YM201636 + 30 min recovery with VBIT-4 vs. 2 h YM201636 and VBIT-4 + 30 min recovery).

    Article Snippet: Erastin (Cat. HY-15763/ MCE), VBIT-4 (Cat. HY-129122/MCE), ML-SI3 (Cat. HY-139426/ MCE), CCCP (Cat. C2759/ Sigma), Nigericin (Cas.

    Techniques: Live Cell Imaging, Transfection, Fluorescence, Expressing